Investigation the effect of Echinophora platyloba and Satureja bachtiarica on MDR1 and ERG11 gene expression in fluconazole resistance clinical isolates Candida albicans using real time PCR

Candida albicans is an opportunist yeast which changes into fungus infection in humans with immunity system deficiency when the required conditions are present. It may cause fatal infections. The Bakhtiari Summer Savoury and Platyloba are anti-fungus plants that effects on MDR 1 and ERG 11 genes expression. This genes have an important role in resistance against Fluconazole.In this study effect of this plants on level expression evaluated by using real time PCR. The ethanolic extract of Savoury and Platyloba were prepared through drying the plants in Percolation method and then 500, 256, 125, 64, 32, 16, 8, 4, 2 and 1 mg/ml concentrations of the solution cocultured with 1×10 clinical isolates of C. albicans after 24 h incubation at 37°C the MIC and MFC were determined. Then C .albicans isolates were treated in MIC concentration of plants. The RNA of isolates were extracted before and after treatment with the plants , then cDNA synthesized and the real timePCR was investigated. The results revealed that the MIC for the alcoholic essences of Satureja and Platyloba were 256 and 64 mg/ml, respectively in the clinical isolates of C. albicans. Also, their MIC and MFC were 512 and 128 mg/ml, respectively. Moreover, the result of expression level of two genes in real-time PCR reaction showed the Platyloba was able to inhibit the ERG11, while the Savoury was neutral in this regard. nowadays, application of the plants with anti-microbial qualities which have less side effects is increasing; because of drug resistances. With regard the present study's findings, the Platyloba is effective in reduction of ERG11 expression which in turn plays a basic role in resistance against Fluconazole. Therefore, the plant may be used as a suitable alternative for drugs and introduced as an efficient anti-fungus factor.